CRISPR-FOCUS: A web server for designing focused CRISPR screening experiments.

CRISPR-FOCUS: A web server for designing focused CRISPR screening experiments.

Blog Article

The recently developed CRISPR screen technology, based on the CRISPR/Cas9 genome editing system, enables genome-wide interrogation of gene functions in an efficient and cost-effective manner.Although many computational algorithms and web servers have been developed to design single-guide RNAs (sgRNAs) with high specificity and efficiency, algorithms specifically designed for conducting CRISPR screens are still lacking.Here we present CRISPR-FOCUS, a web-based platform to search and prioritize sgRNAs for CRISPR screen experiments.

With official gene symbols or RefSeq IDs as the us polo assn mens sweaters only mandatory input, CRISPR-FOCUS filters and prioritizes sgRNAs based on multiple criteria, including efficiency, specificity, sequence conservation, isoform structure, as well as genomic variations including Single Nucleotide Polymorphisms and cancer somatic mutations.CRISPR-FOCUS also provides pre-defined positive and negative control sgRNAs, as well as other necessary sequences in the construct (e.g.

, U6 promoters to drive sgRNA transcription and RNA scaffolds of the CRISPR/Cas9).These features allow users to synthesize oligonucleotides directly based on the output of CRISPR-FOCUS.Overall, CRISPR-FOCUS welding sweater provides a rational and high-throughput approach for sgRNA library design that enables users to efficiently conduct a focused screen experiment targeting up to thousands of genes.

(CRISPR-FOCUS is freely available at http://cistrome.org/crispr-focus/).